How to Run a Cholesterol Assay?

If you’re not a medical practitioner, and you want to learn about cholesterol assay, please visit here about cholesterol test. The blood test examining the levels of cholesterol and fat is also referred to as Lipid Panel.

 Cholesterol Assay

It’s run by a clinic lab professional using Cholesterol Assay Kit. For lab professionals, cholesterol assay kit is an easy and simple measure available. Most of the cholesterol assay kit uses fluorometric method for the sensitive quantitation of total cholesterol in plasma or serum. Cholesterol is hydrolyzed and oxidized to hydrogen peroxide. ADHP(10-acetyl-3,7-dihydroxyphenoxazine) is used as a highly sensitive and stable probe, then horseradish peroxidase catalyzes the ADHP reaction to yield the highly fluorescent product resorufin.  

 

 

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